Brain damage, pulmonary dysfunction, and surfactant derangement during cardiopulmonary bypass for congenital heart diseases. Perioperative parameters and markers to prevent morbidity
Dr. Luca Vedovelli
Inizio progetto – Fine progetto: 01/02/2014-31/01/2017
Budget: 200.000 €
- Manuela Simonato
- Sara D’Aronco
- Giovanna Verlato
- Paola Cogo
Background. As survival has improved in children with congenital heart diseases, morbidity and long-term disability are now considered the key outcome measures.
Project description. Infants < 2 years undergoing cardiac surgery will be prospectively enrolled and divided into 4 groups: Cyanotic, Non-cyanotic, Univentricular heart physiology, and Controls. Venous blood and tracheal aspirates will be collected before and after CPB, and during the cardiac intensive care unit stay (CICU). Neurologic and lung injury markers will be assessed by means of ELISA, liquid chromatography, and mass spectrometry. Stable isotope tracers will be used to investigate surfactant protein metabolism. Clinical and hemodynamic parameters will be also evaluated.
Aims and expected results. Based on preliminary results already acquired, we expect that some differences on lung and brain biomarkers will be found, before and after CPB, and/or among study groups. Thus, the aims of this study are:
1) To assess early biomarkers of brain and lung injury in infants after cardiac surgery and cardiopulmonary bypass (CPB).
2) To correlate the biomarkers with the timing and severity of respiratory and neurologic injury. 3) To build the rationale for perioperative therapies to prevent children morbidity.